Colocalization of Multiple DNA Loci: A Physical Mechanism

Unguided Species Delimitation Using DNA Sequence Data from Multiple Loci

A method was developed for simultaneous Bayesian inference of species delimitation and species phylogeny using the multispecies coalescent model. The method eliminates the need for a user-specified guide tree in species delimitation and incorporates phylogenetic uncertainty in a Bayesian framework. The nearest-neighbor interchange algorithm was adapted to propose changes to the species tree, wi...

Title : A Bayesian Framework for Multiple Trait Colocalization

A unified kinetic mechanism applicable to multiple DNA polymerases.

After extensive studies spanning over half a century, there is little consensus on the kinetic mechanism of DNA polymerases. Using stopped-flow fluorescence assays for mammalian DNA polymerase beta (Pol beta), we have previously identified a fast fluorescence transition corresponding to conformational closing, and a slow fluorescence transition matching the rate of single-nucleotide incorporati...

Construction of a recombinant bacmid DNA containing influenza A virus hemagglutinin gene using a site-specific transposition mechanism

Introduction: In recent years, influenza viruses have caused moderate to severe infections all around the world while so far there is no influenza vaccine that can protect people with only one dose of injection. In this regard, producing a universal vaccine based on virus-like-particles (VLP) could be an ideal approach.  Methods: In this study, the full-length ORF of influenza hemagglutini...

DNA Polymerase : Multiple Conformational Changes in the Mechanism of Catalysis†

Stopped-flow fluorescence assay was applied to identify conformational changes in the catalytic cycle of DNA polymerase (Pol ), using a synthetic DNA primer/template containing 2-aminopurine (2-AP) at the template position opposite the incoming dNTP. Two phases of fluorescence change were observed in the stopped-flow fluorescence assay of the incorporation of the correct nucleotide dTTP. The ra...